Thymosin Beta-4

TB-500 Calculator

For educational purposes only. Not medical advice. Full disclaimer
EV
Elena Vasquez, Biochemistry Updated January 2025

Calculate how to mix and dose TB-500 — no math needed. Just select your vial and dose below.

New to TB-500? Start Here

What is it? TB-500 is a healing peptide (a synthetic version of a protein your body naturally makes) that comes as a freeze-dried powder in a small glass vial.

What does "reconstitution" mean? It just means mixing the powder with water so you can inject it. You add bacteriostatic water (BAC water) — sterile water with a tiny preservative that keeps it safe for weeks.

Loading vs. maintenance? TB-500 uses higher doses at first ("loading phase") to build up levels in your body, then lower doses to maintain them.

What are mcg and mg? Weight measurements: 1 mg (milligram) = 1,000 mcg (micrograms). Your vial is labeled in mg, but doses may be shown in mcg.

TB-500 Protocol Guide

Loading Phase (4-6 weeks):

2-2.5 mg twice weekly

Maintenance Phase:

2-2.5 mg once every 1-2 weeks

TB-500 requires higher doses than BPC-157. Select your vial and dose below.

Check your vial label

Bacteriostatic water — how many ml to add

Per injection

4

Your Results

Concentration

2500

mcg/ml

Draw Per Dose

100

units on syringe

Doses Per Vial

2

total doses

Draw 100 units on a U-100 insulin syringe:

0255075100

Standard 100-unit (1ml) insulin syringe

How TB-500 Reconstitution Works

TB-500 Peptide Powder 5mg vial + Add BAC Water BAC Water 2ml = Mix Ready 2500 mcg/ml Solution 2500 mcg/ml Draw 100 units Draw 100 Units = 2500 mcg dose 2 doses per vial

How to Use This Calculator

  1. Select your vial size – Choose the amount of TB-500 in your vial (2mg, 5mg, or 10mg are common).
  2. Enter BAC water amount – Input how much bacteriostatic water you'll add. Using 2ml keeps calculations simple.
  3. Select your dose – Choose based on your protocol. Loading doses are typically 2-2.5mg, maintenance doses are 1-2mg.
  4. Read your results – The calculator shows your concentration, units to draw, and total doses per vial. Note: higher TB-500 doses mean fewer doses per vial than BPC-157.

About TB-500

TB-500 is a synthetic version of Thymosin Beta-4, a naturally occurring peptide present in almost all human and animal cells. It's a 43-amino acid peptide that has been studied for its role in tissue repair and regeneration.

Research areas include:

  • Wound healing and tissue repair
  • Muscle fiber regeneration
  • Blood vessel formation (helping new blood vessels grow)
  • Reduction of inflammation
  • Hair regrowth

Common TB-500 Protocols

Loading Phase

  • Dose: 2-2.5mg (2000-2500 mcg)
  • Frequency: 2x per week
  • Duration: 4-6 weeks
  • Weekly total: 4-5mg

Maintenance Phase

  • Dose: 1-2mg (1000-2000 mcg)
  • Frequency: 1x per week or every 2 weeks
  • Duration: As needed
  • Weekly total: 1-2mg

* These are commonly discussed protocols. Consult a healthcare professional before use.

Frequently Asked Questions

TB-500 is a synthetic (man-made) version of a small part of Thymosin Beta-4 — a protein your body naturally produces. It contains the "active region" that helps cells move to injury sites and repair tissue. While it's not the full protein, TB-500 is designed to replicate the healing properties that make Thymosin Beta-4 useful.
TB-500 is injected under the skin (called "subcutaneous" injection — not into muscle). Common spots are your belly fat or the front of your thigh. Unlike BPC-157, it doesn't matter if you inject near or far from the injury — TB-500 spreads throughout your whole body.
Many protocols include both TB-500 and BPC-157 together, as they may work through different mechanisms. BPC-157 is often used daily while TB-500 is used 1-2 times per week. They can be injected separately or some mix them in the same syringe.

Done Calculating? Track Your Daily Doses

Set up your protocol once, then come back daily to log injections, monitor vial usage, and stay on schedule.

Open Daily Dose Tracker
BPC-157 Calculator Next: Semaglutide Calculator