Setting Up a BPC-157 and TB-500 Healing Stack Protocol

BPC-157 and TB-500 are the two most frequently combined peptides in healing and recovery research. Each compound works through a different mechanism, and together they address tissue repair from complementary angles. Running both simultaneously requires more planning than a single-compound protocol — you need separate reconstitution math, different dosing frequencies, and a reliable system for tracking two compounds at once.

Why BPC-157 and TB-500 Are Stacked Together

Each peptide contributes something different to the healing process:

• BPC-157 (Body Protection Compound-157) is derived from a protein found in human gastric juice. It promotes localized tissue repair by modulating growth factors, stimulating angiogenesis (new blood vessel formation), and reducing inflammation at the injury site. Its effects are strongest near the injection location, which is why many protocols recommend injecting close to the affected area
• TB-500 (Thymosin Beta-4 fragment) promotes systemic healing by upregulating actin, a cell-building protein involved in cell migration and proliferation. It supports tissue repair throughout the body regardless of injection site, making it effective for widespread inflammation, multiple injury sites, or general recovery

In simple terms: BPC-157 targets local repair and TB-500 supports global healing. The combination addresses both the specific injury site and the overall recovery environment.

Individual Dose Ranges

Each peptide is dosed independently. Do not combine them in the same vial — reconstitute and draw from each one separately.

The most common healing stack starting protocol is BPC-157 at 250 mcg twice daily + TB-500 at 2.5 mg twice weekly. BPC-157 is injected every day, while TB-500 is injected on two fixed days per week (e.g., Monday and Thursday).

Reconstitution Math for Both Compounds

Each peptide is typically sold in 5mg vials. Here is the reconstitution math for the most practical water volumes:

BPC-157 — 5mg Vial + 2mL Bacteriostatic Water

• Concentration: 5,000 mcg / 2 mL = 2,500 mcg per mL
• Per syringe unit: 2,500 / 100 = 25 mcg per unit
• For 250 mcg dose: 250 / 25 = 10 units
• For 500 mcg dose: 500 / 25 = 20 units
• Doses per vial at 250 mcg: 20 doses (10 days at 2x/day)

TB-500 — 5mg Vial + 1mL Bacteriostatic Water

• Concentration: 5,000 mcg / 1 mL = 5,000 mcg per mL
• Per syringe unit: 5,000 / 100 = 50 mcg per unit
• For 2,000 mcg (2mg) dose: 2,000 / 50 = 40 units
• For 2,500 mcg (2.5mg) dose: 2,500 / 50 = 50 units
• Doses per vial at 2.5mg: 2 doses (1 week during loading)

Use less water for TB-500 because the dose is much larger — 2.5mg versus 250 mcg. With 1mL of water, a 2.5mg dose is 50 units, which is a manageable injection volume. Verify your exact syringe measurements with the BPC-157 calculator and TB-500 calculator.

Timing Protocol: When to Inject Each Compound

The daily schedule for this stack involves two injection types with different frequencies:

BPC-157 — Every Day

• Morning dose: Inject 250 mcg subcutaneously, ideally near the injury site
• Evening dose: Inject 250 mcg subcutaneously, 8-12 hours after the morning dose
• BPC-157 has no food timing restrictions — it can be taken with or without meals

TB-500 — Two Fixed Days Per Week

• Choose two days with roughly equal spacing, such as Monday and Thursday or Tuesday and Friday
• Inject 2.5 mg subcutaneously — injection site does not need to be near the injury, as TB-500 works systemically
• Can be taken at the same time as your BPC-157 dose, but drawn from separate vials

Sample Weekly Schedule

4-Week vs. 8-Week Protocol

Protocol length depends on the severity of the injury and the goals of the research:

4-Week Protocol (Standard)

• TB-500 loading for all 4 weeks (2.5 mg, 2x/week)
• BPC-157 daily for all 4 weeks (250 mcg, 2x/day)
• Best for: acute soft tissue injuries, minor tendon or ligament strains, post-procedure recovery

8-Week Protocol (Extended)

• TB-500 loading for weeks 1-4 (2.5 mg, 2x/week), then maintenance for weeks 5-8 (2.5 mg, 1x/week)
• BPC-157 daily for all 8 weeks (250 mcg, 2x/day)
• Best for: chronic injuries, joint issues, more severe tissue damage, or injuries that respond slowly

Vial Planning for the Full Stack

Calculate your total vial needs before starting to avoid running out of either compound mid-protocol:

*8-week TB-500 total assumes 4 weeks loading (20 mg) + 4 weeks maintenance at 2.5 mg 1x/week (10 mg) = 30 mg.

That means a full 4-week healing stack requires approximately 3 BPC-157 vials and 4 TB-500 vials. An 8-week protocol needs roughly 6 of each. Order your full supply before starting and store unreconstituted vials in the freezer for maximum shelf life.

Tracking Two Compounds Daily

Running two peptides simultaneously is where protocol tracking goes from helpful to essential. You are managing different dosing frequencies (daily vs. twice weekly), different vials with different depletion rates, and different reconstitution dates.

The DosageTools Daily Dose Tracker handles multi-compound protocols by letting you log each peptide separately. You can see at a glance which compounds are due today, how many doses remain in each vial, and when each vial was reconstituted. This prevents the most common stacking mistakes:

• Forgetting which day is your TB-500 injection day
• Losing track of which BPC-157 vial you are on
• Missing the fact that your TB-500 supply will run out before your BPC-157 does

Get your exact syringe units for each compound with the BPC-157 dosage calculator and TB-500 dosage calculator.